Canine rabies virus antibody ELISA kit

Canine rabies virus antibody Rapid Assay
1. Summary
This kit use Canine rabies virus solid phase antigen to detect specific antibody against Canine
rabies virus antigen, have high sensitivity, specificity, reproducibility, and is easy to operate quickly
and used repeatedly.
2. Reagents and contents
1. 8× Coated microtiter strips: ready to use
2. 2× Conjugate stock solution: ready to use
3. 2× Conjugate diluent: ready to use
4. 1× PBST powder: ready to use
5. 1× Substrate A solution: ready to use
6. 1× Substrate A solution: ready to use
7. 1× Stopping solution: ready to use
?Warning: Stopping solution irritates eyes and skin. Keep out of the reach of children. Upon
contact with the eyes, rinse thoroughly with water and consult a doctor.?
8. 1× Positive control: ready to use
9. 1× Negative control: ready to use
10. 1× Instruction sheet
3. Test procedure
1. Conjugate solution preparation: add one conjugate stock solution into one conjugate diluent ,
mix thoroughly;
2. Solution preparation: Dissolve each bag PBST powder with 500 ml distilled water,
3. Adding samples and controls: Leave well A1 for reagent blank. Pipette controls and samples as
follows:
Add two drops diluent into each well, then add 5 μl serum sample into each well separately, mix
evenly. Synchronously add critical control and negative control each 100 μl into each two wells
without diluent and add two drops diluent as the blank. incubate in dark at 37 ? for 20 minutes,
then discard the liquid of the well, add 1 drop washing solution into each well and fill with distilled
water promptly, incubate for 30 seconds, then discard the liquid, continue perform another 4
washing cycles as above, then discard the liquid, flap to dry with the absorbent paper.
4. Adding conjugate solution: Dispense 2 drops into all wells (except blank well) and incubate at
37? for 20 minutes. Discard the contents of the wells and wash 5 times as described in step 3.
5. Adding substrates: Dispense 2 drops diluted substrate solution into all wells, incubate at 37? for
10 minutes.
6. Dispense one drop stopping solution into all wells to stop reaction.
4. Results
1 Observation with naked eyes before adding stopping solutionNegative: thickness of color is more thin than critical control
Positive: thickness of color is equal to or more thick than critical control .
2 Judgment by microtiter plate reader
A450 (patient) ≥ critical control: positive
A450 (patient) ? critical control: negative
5. Notes
1 store at 2-8, make the temperature of the kit return to room temperature before use. screw down
the cap after use, don't mix caps between diffirent bottles and don't mix components between
diffirent kits
2 fill wells with distilled water when washing, 30 seconds each time, pour out the liquid. prohibit to
use tap water or any other water.
3 recommand to judge results with instruments to improve comparability of testing.

 

Product Origin: China
Model Number: LSY-30012
Brand Name: GREEN SPRING
   
   

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Company Name: Shenzhen Lvshiyuan Biotechnology Co.,Ltd
Contact Person: Bella Zou
Address:
D building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng,  Shenzhen, China
Zip: 518120
Telephone:
+86-755-28438788
Fax: +86-755-28938800
E-mail: info@lsybt.com
Website: http://lsybtbella.tradeee.com

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