Beta-lactam antibiotics rapid test strip

Beta-lactam antibiotics rapid test strip

 

β-lactam antibiotics rapid test strip  
Catalogue No.: LSY-20044
1. Brief
The β-lactam antibiotics rapid test strip, based on Colloidal gold immunochromatography assay method, is used for testing β-lactam antibiotics residue in milk and goat milk.
2. Specifications
(1) 8 microwells and 8 dipsticks in one bottle      12 bottles / kit.
(2) Instruction                                        1 piece
3. Principle
The β-lactam antibiotics rapid test dipstick is based on competitive inhibition immuno-chromatographic principle. When the β-lactam antibiotics residue in sample is higher than or equal to the detection limit, it will combine with labled antibody, then inhibit the combination between colloidal gold antibody coated antigen on T line, making T line lighter than C line or T line invisible. Otherwise, T line is darker than C line or has same color with C line. No matter whether there is β-lactam antibiotics residue in sample, C line is visible.
 4. Detection limit

β-lactam Name
Detection
Limit (ppb)
β-lactam Name
Detection
Limit (ppb)
Penicillin G
2-4
Cefquinome
8-15
Amoxicillin
2-3
Cefalotin
30-40
Ampicillin
2-3
Cefazolin
40-50
Oxacillin
5-7
Cephalonium
8-10
Cloxacillin
4-6
Ceftriaxone
40-50
Piperacillin
6-8
Ceftiofur
60-100
Nafcillin
20-30
 
 

5. Operation procedures
1) Read the manual carefully before testing, return test dipsticks and milk sample into room temperature before use.
2) Absorb 200ul milk sample into Micro-well, sucking 5~10 times until sample mix even with reagent in micro-well, observe by eyes no solid.
3) Incubate at 40??? for first step, timing for 3min;
4) Insert the dipstick into micro-well, put the end with MAX down, incubate at 40??? for second step, timing for 3min
5) Take out the dipstick from micro-well, judge the result.
6. Test Result Interpretation

Color depth comparison of  T line and C line
Result judgment
Result analysis
T line > C line
Negative
β-lactam antibiotics residue is lower than detection limit in sample
T line = C line
Weak positive
β-lactam antibiotics residue is equal to detection limit in sample
T line < C line or T line is invisible
Positive
β-lactam antibiotics residue is higher than detection limit in sample
C line is invisible
Invalid
The test dipstick is invalid, test again

7. Results picture show
 
 
 
 
 
 
 
 
 
 
8. Precautions
1) This product is suitable for pure milk of cattle and sheep and raw milk.
2) Test dipsticks and micro-wells are disposable,do not use test dipsticks out of date.
3) Please seal the bottle after taking out required test dipsticks. If can not use all 8 microwells at once, cup the rest and put it back to bottle and sealed.
4) Do not touch the white membrane surface in the middle of test dipsticks, avoid sunlight and fan blowing directly.
5) Store milk sample in cold, no more than 3 days.
6) Milk samples should be fully liquid without any agglomeration, sour and precipitation. Stir milk sample to even before test.
7) For milk sample with high fat content, chromatography is slow, recommending extension the second step for another 1 min.
8) Use the test dipsticks testing again for positive results.
9. Specificity
This product has no cross-reaction with Tetracyclines, Quinolones, Sulfonamides, Chloramphenicol and Melamine etc.
 
10. Storage and expiry date
Storage:  Store at 2-8 ??? in sealed, dry, and dark place, no frozen. 
Expiry date: 12 months; date of production is on box.
 
Shenzhen Lvshiyuan Biotechnology Co., Ltd 
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788
Fax 86-755-28938800
Email:  info @lsybt.com
www.lsybt.com

Product Origin: China
Model Number: LSY-20044
Brand Name: GreenSpring
   
   

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Company Name: Shenzhen Lvshiyuan Biotechnology Co.,Ltd
Contact Person: Bella Zou
Address:
D building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng,  Shenzhen, China
Zip: 518120
Telephone:
+86-755-28438788
Fax: +86-755-28938800
E-mail: info@lsybt.com
Website: http://lsybtbella.tradeee.com

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