Doxycycline ELISA test kit

Doxycycline ELISA test kit

 

Doxycycline ELISA Test Kit
Catalog No. LSY-10051
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Doxycycline in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Doxycycline in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti- Doxycycline antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Doxycycline in it. This value is compared to the standard curve and the Doxycycline concentration is subsequently obtained. 
 
2. Technical specifications 
Sensitivity: 2 ppb
Incubation Temperature: 25???
Incubation Time: 30min15min
Detection limit:
Tissueabout 30ppb
Honeyabout 50ppb
Milkabout 100ppb
Note: ppb=ng/mL or ng/g
Cross-reaction rate:
Doxycycline100%
Chlortetracycline400%
Oxytetracyline200%
Tetracycline300%
Recovery rate:
Tissue 90±15%
 
3. Components 
1??? Micro-well strips: 12 strips with 8 removable wells each
2??? 10× concentrated standard solution: 0 ppb, 20ppb, 60ppb, 180ppb540ppb (0.5ml/bottle)
3??? Enzyme conjugate (7 mL)
4??? Antibody working solution (7 mL)
5??? Substrate A (7 mL)
6??? Substrate B (7 mL)
7??? Stop solution (7 mL)
8??? 20× concentrated washing buffer (30 mL)
9??? 20× concentrated redissolving solution (10 mL)  

Product Origin: China
Model Number: LSY-10051
Brand Name: Green Spring
   
   

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Company Name: Shenzhen Lvshiyuan Biotechnology Co.,Ltd
Contact Person: Bella Zou
Address:
D building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng,  Shenzhen, China
Zip: 518120
Telephone:
+86-755-28438788
Fax: +86-755-28938800
E-mail: info@lsybt.com
Website: http://lsybtbella.tradeee.com

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