Ampicillin ELISA Test Kit

Ampicillin ELISA Test Kit

 

Ampicillin ELISA Test Kit
Catalog No. LSY-10018
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Ampicillin in samples. The antigens conjugated Ampicillin is pre-coated on the micro-well stripes, Ampicillin in the sample and the conjugated antigens pre-coated on the micro-well stripes compete for the anti-Ampicillin antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with Ampicillin concentration in the sample. This value is compared to the standard curve and concentration of Ampicillin in the sample is subsequently obtained.
 
2. Technical specifications
Sensitivity: 0.1 ppb
Incubator temperature:  25???
Incubator time:  30min~30min~15min
Detection limit:
Tissue, honey, milk ........................................................................... 2 ppb
Because of some interference, quantification limit of honey, milk sample is 4 ppb
Cross-reaction rate:
Ampicillin........................................................................................... 100%
Benzyl penicillin.................................................................................. 0.8%
Cloxacillin........................................................................................... 0.2%
Dicloxacillin........................................................................................ 0.1%
Amoxicillin.......................................................................................... 0.1%
Ceftiofu.............................................................................................. 0.1%
Recovery rate:
Tissue............................................................................................ 75±19%
Honey, milk.................................................................................... 70±17%
 
3. Components
1???   Micro-well strips: 12 strips with 8 removable wells each
2???   6× standard solution (1 mL each): 0 ppb, 0.1 ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb and 8.1 ppb
3???   Enzyme conjugate (12 mL)............................................................. red cap
4???   Antibody working solution (7 mL).................................................. blue cap
5???   Substrate A solution (7 mL).......................................................... white cap
6???   Substrate B solution (7 mL)......................................................... black cap
7???   Stop solution (7 mL).................................................................. yellow cap
8???   20× concentrated washing buffer (40 mL)..................................... white cap
9???   5× concentrated redissolving solution (50 mL)..................... transparent cap

Product Origin: China
Model Number: LSY-10018
Brand Name: Green Spring
   
   

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Company Name: Shenzhen Lvshiyuan Biotechnology Co.,Ltd
Contact Person: Bella Zou
Address:
D building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng,  Shenzhen, China
Zip: 518120
Telephone:
+86-755-28438788
Fax: +86-755-28938800
E-mail: info@lsybt.com
Website: http://lsybtbella.tradeee.com

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