Zearalenone ELISA test kit

Zearalenone ELISA test kit

 

Zearalenone ELISA test kit
                                                                           Catalog No. LSY-10030
1. General
Zearalenone (ZEN), also called as F-2 toxin, the main component of 2,4 - dihydroxybenzoic acid ester compounds produced by Fusarium graminearum and yellow sickle monocytogenes. ZEN mainly contaminate corn, wheat, grain and other crops, which has a strong estrogen effect, and can cause estrogen pigs, rats, mice, poultry and other animals too much disease and severe symptoms of reproductive tract and infertility, also has immunotoxic, genotoxic etc., it has estrogen effect to human too.
Thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC) has been a common method of Zearalenone detection, but sample preparation and samples analysis of this method is time-consuming and exhausting. The use of Zearalenone ELISA kit to analyze Zearalenone residue of samples is fast and accurate.
This kit is a new generation of fungal toxins detection products in application of ELISA technology, to minimize operating errors and work intensity.
2. Test principle
This test kit is based on the competitive enzyme immunoassay for the detection of Zearalenone in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Zearalenone in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Zearalenone antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Zearalenone in it. This value is compared to the standard curve and the Zearalenone concentration is subsequently obtained.
3. Application
This test kit can be used to detect Zearalenone in grain, beer and feed samples qualitatively and quantitatively.
4. Technical specifications
Sensitivity:  0.2 ppb
Detection limit:
Grain ……………..…………………………………………..…….. 2 ppb
Beer ………………………………………………………..………..2 ppb
Feed …………………………………………………………..……….40 ppb
Cross-reaction rate:
Zearalenone........................................................................... …..100%
α-zearalanol.................................................................................. 75%
β-zeranol...................................................................................... 61%
α-Zearalenol ............................................................................. 103%
β-Zearalenol ............................................................................... 83%
Zearalanone ................................................................................ 75%
5.  Components
1)  ZEN Micro-well strips: 12 strips with 8 removable wells each
2) ZEN 6 x standards ………………………………………2ml each
0 ppb (zero standard), 0.2 ppb, 0.6 ppb, 1.8 ppb, 5.4 ppb, 16.2 ppb
3)  ZEN Enzyme labled conjugate...........................................7 ml
4) Substrate................................................................ ..........12 ml
5)  Stop solution..................................................................... 10 ml
6)  Concentrated wash buffer (10X).........................................50ml
7) Concentrated sample diluent (10X).................................... 20ml

Product Origin: China
Model Number: LSY-10030
Brand Name: Green Spring
   
   

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Company Name: Shenzhen Lvshiyuan Biotechnology Co.,Ltd
Contact Person: Bella Zou
Address:
D building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng,  Shenzhen, China
Zip: 518120
Telephone:
+86-755-28438788
Fax: +86-755-28938800
E-mail: info@lsybt.com
Website: http://lsybtbella.tradeee.com

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