Salbutamol ELISA Test Kit

Salbutamol ELISA Test Kit

 

Salbutamol ELISA Test Kit
Catalog No. LSY-10014
1. Principle
The test kit is based on the competitive enzyme immunoassay for the detection of Salbutamol in the sample. The coupling antigen is pre-coated on the micro-well stripes. The Salbutamol in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Salbutamol antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Salbutamol in it. This value is compared to the standard curve and the Salbutamol residues is subsequently obtained.
 
2. Technical specifications
Sensitivity:  0.1 ppb
Incubator temperature:  25???
Incubator time:  30min???15min
Detection limit
Urine……………………………………………………………..…..…..….0.1 ppb
Tissue (method one)..................................................................... 0.4 ppb
Tissue (method two)...................................................................... 0.1 ppb
Feed............................................................................................... 1 ppb
Recovery rate
    Urine ………………………………………………………………………95%±17%
Tissue.......................................................................................... 75±22%
Feed............................................................................................ 80±18%
Cross-reaction rate
Salbutamol...................................................................................... 100%
Clenbuterol........................................................................................ <1%
Terbutalin.......................................................................................... <1%
Ractopamine................................................................................. < 0.1%
 
3. Components
1???   Micro-well strips: 12 strips with 8 removable wells each
2???   6× standard solution (1 mL each): 0 ppb, 0.1ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb,8.1 ppb
3???   Enzyme conjugate (7 mL)............................................................... red cap
4???   Antibody working solution (10 mL)................................................ blue cap
5???   Substrate A solution (7 mL)......................................................... white cap
6???   Substrate B solution (7 mL)......................................................... black cap
7???   Stop solution (7 mL).................................................................. yellow cap
8???   2× concentrated redissolving solution (50 mL) ……………………..white cap
9???   20x concentrated washing buffer (40 mL) .......................... transparent cap
10???20x Sample extraction solution (50 mL)  ……………….………..yellow cap
 

Product Origin: China
Model Number: LSY-10014
Brand Name: Green Spring
   
   

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Company Name: Shenzhen Lvshiyuan Biotechnology Co.,Ltd
Contact Person: Bella Zou
Address:
D building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng,  Shenzhen, China
Zip: 518120
Telephone:
+86-755-28438788
Fax: +86-755-28938800
E-mail: info@lsybt.com
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